Bob Weinberg) with BamHI (overhang removed) and XbaI (overhang filled). * A Research Technician II may serve as a team leader to lab assistants and Research Technician I's. Backbone manufacturer 42230 Backbone size (bp) 8506 Modifications to backbone NotI site cleaved and filled, SV40-Puro cassete from pBABE.puro (Addgene 1764 from Dr. SUPERVISORY RESPONSIBILITY (if applicable): * Those with a minimum of 1 year of directly related work experience will be considered for a Research Technician II position. * New graduates with some lab experience (via course work, internships, etc.) or those without any prior research experience will be considered for the Research Technician I position outlined above. * Must have a positive attitude and willingness to learn and grow * Demonstrate honesty, integrity and reliability * Ability to perform multiple tasks independently A few other regions were suspected to encode proteins, but they had not been definitively classified as protein-coding genes. Scientists have identified several regions known to encode protein-coding genes, based on their similarity to protein-coding genes found in related viruses. * Must communicate regularly and effectively with team members and supervisor The SARS-CoV-2 genome consists of nearly 30,000 RNA bases. * Ability to work independently and as a team member * Exceptional organizational skills and excellent attention to details * Proficiency and/or demonstrated ability to learn bioinformatics software platforms such as Geneious Prime, Snapgene, and R * Maintain detailed lab notebook documenting all experimental work dna into dict, json, and biopython object. * Capable of presenting data at internal lab meetings and conferences A Python project to read and write Snapgene. * Experience with library preparation highly desirable * Experience with standard molecular biology techniques such as DNA/RNA isolation, standard and real time PCR required Similarly, not all duties that have been outlined will be assigned to each position. Specific responsibilities may vary based upon departmental needs. Please note, the functions below are representative of major duties that are typically associated with these positions. The Research Technician will be part of a dynamic team working closely with postdoctoral researchers, under the supervision of a Research Scientist, to ensure high-quality and timely delivery of results. The position offers exceptional opportunity for scientific growth in a fast-paced lab working at the interface of basic B cell biology and preclinical vaccinology. For standard Gateway cloning, a DNA fragment is inserted into a Donor Vector in a BP cloning reaction, creating an. The selected candidate will join a dynamic research team at the forefront of B cell immunology to contribute to Next Generation Sequencing (NGS) projects, with an emphasis on library construction for 10x Genomics single-cell sequencing. SnapGene simulates different variations of this method.
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